中国食品添加剂

为了确定当归药材中当归多糖成分的提取、分离纯化工艺及免疫调节功能，以当归药材中多糖的提取效率为考察指标，通过单因素实验和正交实验考察当归药材中多糖的提取工艺条件，并对当归药材中多糖进行纯化，考察纯化后不同当归多糖组分的免疫调节功能。结果显示当归多糖最佳提取工艺为：当归药材粉碎，加入3%的纤维素酶和150倍的纯水，柠檬酸调节pH值为5,50℃下提取2.5 h，当归多糖的提取率为（36.71±0.38）%。提取物分离纯化得到不同当归多糖组分APS-1和APS-2，免疫调节实验结果显示APS-1和APS-2均能促进斑马鱼巨噬细胞吞噬作用，两种组分的起效浓度均在50μg/mL左右；因此，本研究的方法对当归多糖提取纯化效果较好，获得的当归多糖组分具有较好的免疫调节功能，为当归多糖提取纯化和功能研究提供数据支撑。

In order to optimize the extraction and purification process of Angelica sinensis polysaccharide and evaluate their immune regulation functions. The extraction yield of Angelica polysaccharide was used as the primary index. The extraction conditions were optimized via single-factor and orthogonal experiments,followed by purification of the extracts. The immunoregulatory effects of different purified polysaccharide fractions were then investigated. The results showed that the optimal extraction process was as follows :crushing Angelica sinensis,adding 3% cellulase and 150 times pure water,adjusting the pH to 5 with citric acid,and extracted at 50 ℃ for 2.5 h. Under the above conditions,the polysaccharide extraction yield was( 36.71±0.38)%. The crude polysaccharide was separated and purified to obtain two main polysaccharide fractions :APS-1 and APS-2. Immunomodulatory assays demonstrated that both APS-1 and APS-2 significantly enhanced the phagocytic activity of zebrafish macrophages,with the effective concentration was about 50 μg/mL. Thus,the method employed in this study was demonstrated to efficiently extract and purify Angelica polysaccharides,The obtained Angelica polysaccharide fractions demonstrated notable immunomodulatory activity,providing data support for the extraction,purification and functional research of Angelica polysaccharide in the future.