中国食品添加剂

本研究建立了一种适用于可食用油墨中甘油含量测定方法，以期为可食用油墨的质量控制与安全监管提供可靠的技术支撑。实验方法如下：将样品用纯水超声提取，通过CNW BOND HLB固相萃取柱净化，收集上样液及淋洗液，氮吹，89%乙腈复溶，以VC-HILIC色谱柱为分析柱，蒸发光散射检测器分析，等度洗脱，外标法定量。结果显示：在0.4～1.2 mg/mL范围内对数工作曲线相关系数大于0.99，方法检出限为0.25g/kg，定量限为0.50 g/kg；分别向阴性样品中添加浓度为定量限、二倍定量限、十倍定量限浓度的标准溶液，其平均加标回收率为81.09%～106.16%，相对标准偏差为3.53%～9.65%。本研究建立的方法简单、快速，能广泛应用于不同类型食用油墨中甘油含量的分析检测。

A method for determining glycerol content in edible inks was established,aiming to provide reliable technical support for quality control and safety supervision of edible inks. The experimental method was as follows :samples were extracted by ultrasonication with pure water,purified using a CNW BOND HLB solid-phase extraction column,and the loading and elution solutions were collected. The solvent was removed by nitrogen blowing,and the residue was reconstituted in 89% acetonitrile. Analysis was performed using a VC-HILIC column with an evaporative light scattering detector(ELSD) under isocratic elution conditions,and quantification was carried out using the external standard method. The results showed that in the range of 0.4 ～ 1.2 mg/mL,the correlation coefficient of the logarithmic working curve was greater than 0.99,the limit of detection of the method was 0.25 g/kg,and the limit of quantification was 0.50 g/kg;the average recovery of the standard solution spiked at the limit of quantification(LOQ),2×LOQ and 10×LOQ was 81.09% ～ 106.16%,and the relative standard deviation was 3.53% ～ 9.65%. The established method was simple and rapid,and could be widely used for the determination of glycerol in different types of edible ink.