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本研究旨在探究天麻多酚的最优提取工艺及其体外活性。以新鲜天麻为原料，先通过单因素试验分析影响天麻多酚提取效率的因素，再用响应面优化试验法确定最优提取工艺。研究发现，超声辅助纤维素酶法提取天麻多酚的最佳提取工艺为：液料比31:1、浓度73%的乙醇、纤维素酶1.3%、超声功率290 W，此时天麻多酚的提取得率为0.62%。体外活性研究表明，天麻多酚提取物清除ABTS+自由基、DPPH自由基、羟自由基的半抑制浓度(IC50)分别为43.44、65.75、31.15μg/mL。在降糖能力方面，其对α-淀粉酶和α-葡萄糖苷酶的IC50值分别为41.29、22.40μg/mL。本研究成果可为超声辅助纤维素酶法提取天麻多酚的工艺及体外活性研究提供参考。
Abstract：This study aimed to optimize the extraction process of Gastrodia elata polyphenols and evaluate their bioactivities in vitro. Fresh G. elata was used as the raw material. Single-factor experiments were first conducted to examine the key variables influencing polyphenol extraction efficiency. Subsequently,response surface methodology was applied to determine the optimal conditions for ultrasonic-assisted cellulase extraction. The optimized extraction parameters were :liquid-to-material ratio of 31:1,ethanol concentration of 73%,cellulase content of 1.3%,and ultrasonic power of 290 W,resulting in a polyphenol extraction rate of 0.62%. In vitro activity assays revealed that the half-maximal inhibitory concentrations(IC50) for ABTS+,DPPH,and hydroxyl radicals were 43.44,65.75,and 31.15 μg/mL,respectively. Regarding glucose-lowering activity,the IC50 values for α-amylase and α-glucosidase inhibition were 41.29 and 22.40 μg/mL,respectively. These findings provided a useful reference for the efficient extraction and in vitro activity evaluation of G. elata polyphenols using ultrasound-assisted cellulase methods.
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基本信息:
DOI：10.19804/j.issn1006-2513.2025.11.003
中图分类号:TS201.2
引用信息:
[1]盛婧,许美铃,董仕豪,等.超声辅助纤维素酶法提取天麻多酚的工艺优化及体外活性研究[J].中国食品添加剂,2025,36(11):16-25.DOI:10.19804/j.issn1006-2513.2025.11.003.
基金信息:
云南省大学生创业训练项目（S202410677100）; 云南省天麻与真菌共生生物学重点实验室开放课题（TMKF2024B05）