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建立一种基于胃蛋白酶能促进消化的机理的高通量筛选方法，以便为快速筛选具有促进消化功能的中草药提供筛选依据。以酪氨酸为阳性对照，采用多功能酶标仪及微孔板为载体，通过反应条件优化，对200种中药提取液进行筛选评价。200种中药材中清热药、解表药中高活性的比例较高；而补益药、矿物药多表现出较低的活性。基于胃蛋白酶的促进作用建立的高通量筛选方法具有稳定、高效、低成本，可用来快速筛选中药材及天然成分，为中药材促进消化功能的研发提供参考。
Abstract：A high-throughput screening method based on the promoting effect of pepsin was established to provide a scientific basis for rapid identification of digestion-enhancing Chinese herbal medicines. Using tyrosine as the positive control,a multifunctional microplate reader and 96-well microplates were employed as the experimental platform. After optimization of reaction conditions,200 extracts from Chinese medicinal herbs were systematically screened and evaluated. Among these 200 species,heat-clearing herbs and exterior-resolving herbs exhibited higher proportions of high activity,while tonifying herbs and mineral-based herbs predominantly showed low activity. The established screening method demonstrated stability,high efficiency,and cost-effectiveness,making it suitable for rapid screening of Chinese medicinal herbs and natural compounds. This approach provides a valuable reference for the development and research of herbal medicines targeting digestive function improvement.
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基本信息:
DOI：10.19804/j.issn1006-2513.2026.8.003
中图分类号:R284.1
引用信息:
[1]周俊强,崔灵敏,赖富丽,等.基于胃蛋白酶消化作用的高通量筛选方法建立及其对200种中药材的活性评价[J].中国食品添加剂,2026,37(08):28-36.DOI:10.19804/j.issn1006-2513.2026.8.003.
2026-08-04
2026-08-04
2026-08-04