中国食品添加剂

本研究以石榴皮和百香果果皮为原料，进行石榴皮及百香果果皮（Pomegranate and passion fruit peel,PPFP）复合发酵饮料的研制，通过单因素及响应面试验优化复合饮料发酵工艺条件，并探讨其对小鼠运动耐力的影响。结果表明：复合饮料最佳发酵工艺条件为：石榴皮汁添加量20%、百香果果皮汁添加量24%、发酵时间36 h、发酵温度40℃，感官评分为（94.05±1.85）分，活菌数为（8.5±0.2） lg(CFU/g)，在此发酵工艺条件下研制的复合饮料色泽均一，具有石榴皮混合发酵后的独特风味，酸甜适口，无明显分层现象。小鼠试验结果表明：与空白对照组相比，低、中、高剂量组可显著延长小鼠负重力竭游泳时间，小鼠肝糖原和肌糖原储备显著增加，显著降低小鼠血清中乳酸和尿素氮含量（P<0.05）。说明PPFP复合发酵饮料有利于提高小鼠机体运动耐力。

Using pomegranate peel and passion fruit peel as raw materials,a fermented pomegranate peel and passion fruit peel compound beverage was developed. The fermentation process conditions of the pomegranate peel and passion fruit peel compound beverage were optimized through single factor and response surface methodology experiments,and its effect on the exercise endurance of mice was explored. The results showed that the optimal fermentation conditions for the pomegranate peel and passion fruit peel compound beverage were :20% pomegranate peel juice added,24% passion fruit peel juice added,fermentation time of 36 h,fermentation temperature of 40 ℃,sensory score of(94.05±1.85) points,and live bacterial count of(8.5±0.2) lg(CFU/g). Under these fermentation conditions,the developed pomegranate peel and passion fruit peel compound beverage had a uniform color and unique flavor after mixed fermentation of pomegranate peel and passion fruit peel,with a sweet and sour taste,and without obvious layering phenomenon. The results of the mice experiment showed that,compared with the blank control group,the low,medium,and high dose groups could significantly prolong the weight exhaustion swimming time of mice,significantly increase mice liver glycogen and muscle glycogen reserves,and significantly reduce mice serum lactate and urea nitrogen content(P<0.05). It indicated that the fermented pomegranate peel and passion fruit peel compound beverage was beneficial for improving the exercise endurance of mice.