中国食品添加剂

建立一种可同时测定多种食品基质中7种甜菊糖苷类化合物的高效液相色谱（HPLC）检测方法。选取饮料、配制酒、腌渍蔬菜、调味品及水果罐头5类代表性食品为研究对象，系统考察不同样品基质对7种甜菊糖苷类化合物色谱行为的影响。通过优化色谱柱、流动相组成及梯度洗脱程序，消除基质干扰，实现7种甜菊糖苷类化合物的有效分离。采用所建方法对5类食品共15份样品进行一水平加标回收试验，验证方法的准确性与适用性。结果显示采用乙腈–磷酸二氢钠溶液为流动相，经梯度洗脱优化后，7种甜菊糖苷均可获得良好的基线分离与峰形。15份供试样品的加标回收率均在90%～110%，符合GB/T 27404—2026的相关要求。该方法操作简便、准确可靠，具有良好的基质适用性和重现性，可满足多种食品基质中甜菊糖苷类化合物的定性筛查与定量检测需求。

A high-performance liquid chromatography(HPLC) method was developed for the simultaneous determination of seven steviol glycosides in various food matrices. Five representative food categories,namely beverages,prepared wines,pickled vegetables,condiments,and canned fruits,were selected for simultaneous detection of seven steviol glycosides. Effects of different sample matrices on the chromatographic behavior of seven steviol glycosides was systematically investigated. By optimizing the chromatographic column,mobile phase composition,and gradient elution program,matrix interference was eliminated,achieving effective separation of seven steviol glycosides. The established method was applied to a one-level spiked recovery experiment using 15 samples across the five food categories to verify its accuracy and applicability. With acetonitrile-sodium dihydrogen phosphate solution as the mobile phase,the optimized gradient elution achieved baseline separation for all seven steviol glycosides,yielding good peak shapes. The spiked recoveries for the 15 samples ranged from 90% to 110%,complying with the requirements of GB/T 27404-2026. The developed method was simple,accurate,and reliable,demonstrating good matrix applicability and reproducibility. It can effectively meet the requirements for qualitative screening and quantitative determination of steviol glycosides in diverse food matrices.