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原花青素是广泛存在于植物体内的一大类多酚化合物,在酸性条件下加热会生产花青素。原花青素具有极高的抗氧化性和自由基清除能力,还有消炎抗癌、抗衰老等作用,因此广泛应用于医药、食品、保健和化妆品等方面。葡萄籽中含有大量的优质原花青素,通过乙醇浸提法提取葡萄籽中的原花青素,既可以充分利用葡萄酒副产物,又可以生产优质原花青素。以原花青素提取量和DPPH自由基清除能力为试验指标确定乙醇法提取葡萄籽原花青素的最佳工艺条件,为原花青素的进一步开发和利用提供支持。经过单因素试验与正交试验的反复探索得出葡萄籽原花青素提取的最佳工艺条件为:料液比1∶10,提取时间90 min,提取温度20℃,乙醇浓度70%,pH=4,经验证实验确定原花青素提取量为1.584 mg/g,DPPH自由基清除能力为12.41μmol/g。
Abstract：Proanthocyanidins is major polyphenols and widely existed in plants,and will produce anthocyanin under heating in acidic conditions. Proanthocyanidins has a very high resistance to oxidation and strong radical scavenging ability,and it also has anti-inflammatory,anti-cancer,anti-aging functions. Therefore,it widely used in medicine,food,health care and cosmetics. There are abundant proanthocyanidins in grape seeds,extracting proanthocyanidins from grape seeds by ethanol can fully use of the wine making by-products. The extraction rate of proanthocyanidins and its DPPH free radical scavenging capacity are two indexes in determining the optimum extraction process. The experiment provides support for the further development and utilization of proanthocyanidins. The optimum extraction process conditions determined by single-factor and orthogonal test are as follows:solid-liquid ratio of 1∶10,extraction time 90 min,extraction temperature 20℃,ethanol concentration 70%,pH=4. Under the above conditions,the extraction amount of proanthocyanidins was 1.584mg/g,and DPPH free radical scavenging capacity was 12.41 μmol/g.
[1]李超,王卫东.原花青素提取方法的研究进展[J].粮油加工.2009(9):145-148.
[2]姚方耀.葡萄籽中葡萄籽油和原花青素的提取分离工艺研究[D].天津:天津大学,2004.
[3]Huh Y S,Hong T H,Hong W H.Effective extraction of oligomeric proanthocyanidin from wild grapeseeds(OPC)[J].Journal of Biotechnology and Bioprocess Engineering,2004,9(6):471-475.
[4]Escarpa A,Gonzalezm C.Identification and quantitation of phenolics from green beans by high performance Liquid chromatography[J].Chromatographia,2000,52(12):33-38.
[5]孙来华.葡萄籽功能成分及其应用[J].食品研究与开发,2011,32(7):184-188.
[6]Bagchi D,Swaroop A,Preuss H G,et al.Free radical scavenging,antioxidant and cancer chemoprevention by grape seed proanthocyanidin[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,2014,768:69-73.
[7]Cádiz-Gurrea M D L L,Borrás-Linares I,Lozano-Sánchez J,et al.Cocoa and Grape Seed Byproducts as a Source of Antioxidant and Anti-Inflammatory Proanthocyanidins[J].International Journal of Molecular Sciences.2017,18(2):376.
[8]Nunes M A,Pimentel F,Costa A S G,et al.The integrated antioxidant system[J].Trends in Food Science&Technology,2016,57:31-39.
[9]Ahmad S F,Zoheir K M A,Abdel-Hamied H E,et al.Grape Seed Proanthocyanidin Extract Protects Against CarrageenanInduced Lung Inflammation in Mice Through Reduction of Proinflammatory Markers and Chemokine Expressions[J].Journal of Food Science,2014,37(2):500-511.
[10]Wang H,Xue Y S,Zhang H Y,et al.Dietary grape seed extract ameliorates symptoms of inflammatory bowel disease in IL10-deficient mice[J].Molecular Nutrition&Food Research,2013,57(12):2253-2257.
[11]Khan N Q,Patel B,Kang S S,et al.Regulation of vascular endothelial function by red wine procyanidins:implications for cardiovascular health[J].Tetrahedron,2015,71(20):3059-3065.
[12]Valls R M,LlauradóE,Fernández-Castillejo S,et al.Effects of low molecular weight procyanidin rich extract from french maritime pine bark on cardiovascular disease risk factors in stage-1 hypertensive subjects:Randomized,double-blind,crossover,placebo-controlled intervention trial[J].Phytomedicine,2016,23(12):1451-1461.
[13]Yang H X,Xiao L,Yuan Y,et al.Procyanidin B2inhibits NLRP3 inflammasome activation in human vascular endothelial cells[J].Biochemical Pharmacology,2014,92(4):599-606.
[14]张若冰,劳业兴,苏薇薇.原花青素的研究现状及开发前景[J].中药材,2003,26(12):905-908.
[15]Preur C,Rigaud J,Cheynier V,et al.Micro RNA-19a/b mediates grape seed procyanidin extract-induced antineoplastic effects against lung cancer[J].Photochemistry,2016,34:118-125.
[16]Fernández-Iglesias A,Quesada H,Díaz S,et al.Combination of grape seed proanthocyanidin extract and docosahexaenoic acid-rich oil increases the hepatic detoxification by GST mediated GSH conjugation in a lipidic postprandial state[J].Food Chemistry,2014,165(12):14-20.
[17]范明远,叶音.体内自由基清除剂及抗氧化剂-原花青素的研究进展[J].中国预防医学杂志,2001,2(4):303-305.
[18]叶孝兆,周亮,莫启武,姚煜东.超临界萃取葡萄籽原花青素的研究[J].实验与技术.2007(10):20-21.
[19]邵丽丽,刘志杰.原花青素的研究[J].科技论坛2009(5):34-35.
[20]王亮,张文胜.原花青素功能与性质的研究工艺[J].广东轻工职业技术学院学报.2006(3):34-35.
[21]王佳宁,郜玉钢,臧埔等.原花青素的提取方法与药理作用研究进展[J].上海中医药杂志.2015,49(10):94-97.
[22]王桥,宋学英,朱莹,等.葡萄提取物抗氧化与抗缺氧作用的研究[J].2003(6):50-51.
[23]Fortes G A C,Carvalho A G,Ramalho R R F,et al.Antioxidant Activities of Hydrolysable Tannins and Flavonoid Glycosides Isolated from Eugenia uniflora L.[J].Records of Natural Products,2015,9(2):251-256.
[24]Jara-Palacios M J,Hernanz D,Escudero-Gilete M L,et al.The Use of Grape Seed Byproducts Rich in Flavonoids to Improve the Antioxidant Potential of Red Wines[J].Molecules,2016,21(11),1526.
[25]任小娜,迪丽努尔,陈志梅.黑果枸杞中原花青素稳定性和抗氧化性研究[J].湖北农业科学,2016,55(14):3697-3700.
[26]陆书玉.环境影响评价[M].北京:高等教育出版社,2002:163-164.
[27]Brandwilliams W,Cuvelier M E,Berset C.Use of a f reeradical method to evaluate antioxidant acitivity[J].Food Science and Technology-Lebensmittel-Wissenschaft and Technologe,1995,28(1):25-30.
基本信息:
中图分类号:TQ914.3
引用信息:
[1]李丹丹,梁英,杨宏志,等.葡萄籽原花青素提取工艺的研究[J].中国食品添加剂,2017,No.162(08):55-62.
基金信息:
国家自然科学基金青年科学基金项目(3101828);; 大庆市指导性科技计划项目(zd-2016-108);; 黑龙江八一农垦大学引进人才科研启动计划资助项目(XYB2013-16)