| 79 | 0 | 29 |
| 下载次数 | 被引频次 | 阅读次数 |
本试验建立了一种利用离子色谱(HPAEC)同时测定婴幼儿配方奶粉中六种母乳低聚糖(HMOs)含量的方法，该方法通过磺基水杨酸溶液沉淀样品中的蛋白质，考察HMOs及麦芽糊精、低聚果糖(FOS)、低聚半乳糖(GOS)、乳糖在PA20和PA1色谱柱中的分离度与干扰情况，随后经分析方法和样品前处理优化后，最终选择PA1色谱柱进行分离。在优化条件下，六种HMOs在其线性范围内线性关系良好，加标回收率为90%~105%，检出限为0.0120.168 g/100g，定量限为0.0401.640 g/100g，本方法具有灵敏度高、成本较低的特点，能够满足婴幼儿配方奶粉中六种HMOs的检测需求。
Abstract：A method for simultaneously determining the content of six human milk oligosaccharides(HMOs) in infant formula using High-Performance Anion-Exchange Chromatography(HPAEC) was established. Proteins in the samples were precipitated with sulfosalicylic acid solution,and the separation and potential interferences of HMOs,maltodextrin,fructooligosaccharides(FOS),galactooligosaccharides(GOS) and lactose on PA20 and PA1 columns were examined. Following the analytical method and sample pretreatment optimization,the PA1 column was ultimately selected for final separation. Under the optimized conditions,the six human milk oligosaccharides showed good linearity within their respective calibration ranges,the recoveries of the spiked samples ranged from 90%~105%,the limits of detection(LOD) were 0.0120.168 g/100g,and the limits of quantification(LOQ) were 0.0401.640 g/100g. The method was demonstrated to be sensitive,cost-effective and proving suitable for the determination of six HMOs in infant formula.
[1]中国食品科学技术学会.母乳低聚糖（HMOs）的科学共识[J].中国食品学报，2023,23(6):452-457.
[2]Sischo W M,Short D M,Geissler M,et al. Comparative composition,diversity,and abundance of oligosaccharides in early lactation milk from commercial dairy and beef cows[J].Journal of Dairy Science,2017,100(5):3883-3892.
[3]van Leeuwen S S,te Poele E M,Chatziioannou A C,et al. Goat milk oligosaccharides:Their diversity,quantity,and functional properties in comparison to human milk oligosaccharides[J]. Journal of Agricultural and Food Chemistry,2020,68(47):13469-13485.
[4]史玉东，刘梦瑶，卢卫红.母乳低聚糖的结构与功能研究进展[J].食品安全质量检测学报，2020, 11(21):7656-7662.
[5]国家卫生健康委.关于桃胶等15种“三新食品”的公告[EB/OL].(2023-09-22)[2025-02-03]. https：//zwfw.nhc.gov.cn/kzx/tzgg/sptjjxpzsp_224/202310/t20231018_2617.html.
[6]Zhu Y Y,Cao H Z,Wang H,et al. Biosynthesis of human milk oligosaccharides via metabolic engineering approaches:Current advances and challenges[J]. Current Opinion in Biotechnology,2022,78:102841.
[7]Kunz C,Rudloff S,Baier W,et al. Oligosaccharides in human milk:Structural,functional,and metabolic aspects[J]. Annual Review of Nutrition,2000,20:699-722.
[8]李欣.人乳寡糖的分离制备及其在婴儿配方粉中的含量分析研究[D].大连：大连医科大学，2020:35-45.
[9]Ellingson D J,Ruosch A J,Foster K L,et al. Analysis of six human milk oligosaccharides(HMO)in infant formula and adult nutritionals by 2AB labeling and quantification with HILIC-FLD:First action 2022.02[J]. Journal of AOAC INTERNATIONAL,2022,106(1):112-126.
[10]Christensen A S,Skov S H,Lendal S E,et al. Quantifying the human milk oligosaccharides 2’-fucosyllactose and3-fucosyllactose in different food applications by highperformance liquid chromatography with refractive index detection[J]. Journal of Food Science,2020,85(2):332-339.
[11]喻斌斌，叶文慧，刘彪.阴离子交换色谱测定乳粉中2’-岩藻糖基乳糖[J].食品工业，2022,43(2):304-307.
[12]卢鑫，朱桂芳.离子色谱法测定婴幼儿配方食品中的2’-岩藻糖基乳糖[J].实验室检测，2023(6):1-7.
[13]Austin S,Cuany D,Michaud J,et al. Determination of2’-fucosyllactose and lacto-N-neotetraose in infant formula[J]. Molecules,2018,23(10):2650.
[14]Haselberger P,Tian F,Erney R,et al. Method for t h e d e t e r m i n a t i o n o f 2 ’-f u c o s y l l a c t o s e(2 ’-F L),3-fucosyllactose(3-FL),6’-sialyllactose(6’-SL),3’-sialyllactose(3’-SL),lacto-N-tetraose(LNT),and lacto-N-neoTetraose(LNnT)by high-performance anionexchange chromatography with pulsed amperometric detection(HPAEC-PAD):First action 2022.04[J]. Journal of AOAC INTERNATIONAL,2023,106(5):1237-1245.
[15]Ma L,McJarrow P,Fong B Y. Quantification of major milk oligosaccharides in a range of formulated milk powder products using high performance liquid chromatography-multi reaction monitoring-mass spectrometry[J]. International Dairy Journal,2019,94:1-6.
[16]Liu F,van der Molen J,Kuipers F,et al. Quantitation o f b i o a c t i v e c o m p o n e n t s i n i n f a n t f o r m u l a s:M i l k oligosaccharides,sialic acids and corticosteroids[J]. Food Research International,2023,174:113589.
[17]Fong B, Ma K, McJarrow P. Quantification of bovine milk oligosaccharides using liquid chromatography–selected reaction monitoring–mass spectrometry[J]. Journal of Agricultural and Food Chemistry, 2011, 59(18):9788-9795.
[18]Nijman R M,Liu Y,Bunyatratchata A,et al.Characterization and quantification of oligosaccharides in human milk and infant formula[J]. Journal of Agricultural and Food Chemistry,2018,66(26):6851-6859.
[19]Galeotti F,Coppa G V,Zampini L,et al. Capillary electrophoresis separation of human milk neutral and acidic oligosaccharides derivatized with 2-aminoacridone[J].ELECTROPHORESIS,2014,35(6):811-818.
[20]Olivares M, Albrecht S, De Palma G, et al. Human milk composition differs in healthy mothers and mothers with celiac disease[J]. European Journal of Nutrition, 2015, 54(1):119-128.
[21]van Leeuwen S S,Schoemaker R J W,Gerwig G J,et al.Rapid milk group classification by 1H NMR analysis of Le and H epitopes in human milk oligosaccharide donor samples[J].Glycobiology,2014,24(8):728-739.
[22]Auer F,Jarvas G,Guttman A. Recent advances in the analysis of human milk oligosaccharides by liquid phase separation methods[J]. Journal of Chromatography B,Analytical Technologies in the Biomedical and Life Sciences,2021,1162:122497.
[23]Thurl S,Muller-Werner B,Sawatzki G. Quantification of individual oligosaccharide compounds from human milk using high-pH anion-exchange chromatography[J]. Analytical Biochemistry,1996,235(2):202-206.
[24]Bao Y W,Zhu L B,Newburg D S. Simultaneous quantification of sialyloligosaccharides from human milk by capillary electrophoresis[J]. Analytical Biochemistry,2007,370(2):206-214.
[25]陈磊，Philip Haselberger，田芳，等.离子色谱法测定母乳中的寡聚糖与游离唾液酸[J].中国食品学报，2019,19(10):227-234.
[26]Urashima T,Saito T,Ohmisya K,et al. Structural determination of three neutral oligosaccharides in bovine(Holstein-Friesian)colostrum,including the novel trisaccharide ;GalNAc alpha 1-3Gal beta 1-4Glc[J].Biochimica et Biophysica Acta,1991,1073(1):225-229.
基本信息:
DOI：10.19804/j.issn1006-2513.2026.3.014
中图分类号:TS252.51;O657.7
引用信息:
[1]王弈忻,李培功,陈楠楠,等.HPAEC法测定婴幼儿配方奶粉中六种母乳低聚糖[J].中国食品添加剂,2026(03):113-129.DOI:10.19804/j.issn1006-2513.2026.3.014.
基金信息:
国家乳业技术创新中心项目(2022-开放性课题-23); 北京市科技计划项目(Z231100003723014)
2025-09-06
2025
2025-11-12
2025-11-13
2025
1
2026-02-26
2026-02-26
2026-02-26