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本研究建立了复配营养强化剂中维生素D3超高效液相色谱检测方法。样品加入氨水，于55℃超声波水浴中处理，冷却后加入三氯甲烷、乙醇提取维生素D3，离心分离，取上清液用0.22μm有机系滤膜过滤，采用ACQUITY UPLC HSS T3色谱柱分离，流动相为甲基叔丁基醚、甲醇、0.1%甲酸溶液，梯度洗脱，紫外检测器采集数据，外标法定量。对方法进行验证，维生素D3在其质量浓度范围内线性关系良好，标准曲线的R2 > 0.99，回收率为91.0%～102.0%，相对标准偏差为2.29%～3.52%，检出限为0.02μg/g。该方法快速、准确，适合各类复配营养强化剂中维生素D3的测定。
Abstract：An ultra-high performance liquid chromatography(UHPLC) method was established for the determination of vitamin D3 in compound nutritional fortifiers. Samples were treated with ammonia in an ultrasonic water bath at 55 ℃. After cooling,vitamin D3 was extracted using a mixture of trichloromethane and ethanol. Chromatographic separation was performed on an ACQUITY UPLC HSS T3 column using a mobile phase of methyl tert-butyl ether,methanol,and 0.1% formic acid. Detection was conducted using a UV detector and quantified using the external standard method. The method demonstrated excellent linearity(R2>0.99),with recoveries ranging from 91.0% to 102.0% and relative standard deviations(RSD) between 2.29% and 3.52%. The limit of detection(LOD) was 0.02 μg/g. This method was rapid,accurate,and suitable for the routine analysis of vitamin D3 in various compound nutritional fortifiers.
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基本信息:
DOI：10.19804/j.issn1006-2513.2026.7.015
中图分类号:O657.72;TS202.3
引用信息:
[1]王剑飞,王鑫,于宁,等.超高效液相色谱法检测复配营养强化剂中维生素D_3[J].中国食品添加剂,2026(07):117-122.DOI:10.19804/j.issn1006-2513.2026.7.015.
2026-07-06
2026-07-06
2026-07-06