中国食品添加剂

瓦尼桑黄是一种珍惜的药食两用资源，但是其野生资源较为稀少，液体发酵是桑黄扩大培养的方法之一。本文通过单因素试验和正交试验，以桑黄多糖产量为标准，优化桑黄多糖的液体发酵条件为：pH值7.0，装液量120 mL，接种量10.0%，转速为150 r/min，培养箱28℃培养9 d。通过正交试验考察了料液比、浸提时间、提取温度及超声功率对多糖提取率的影响，得到最佳提取工艺参数：料液比1∶75(g/mL)，提取温度80℃，提取时间60 min，多糖提取率达到5.7%。

Sanghuangporus vaninii is a precious medicinal and edible resource,but its wild resources are relatively rare. Liquid fermentation is a key method for its large-scale expansion. This study aimed to optimize the liquid fermentation and subsequent extraction processes for maximizing the yield of S. vaninii polysaccharide. The fermentation conditions were optimized using single-factor and orthogonal experiments with polysaccharide yield as the primary index. Following fermentation,the polysaccharide extraction process was optimized using an orthogonal design,investigating the effects of solid-liquid ratio,extraction time,extraction temperature,and ultrasound power. The optimal liquid fermentation conditions were as follows :pH 7.0,liquid volume 120 mL in 250 mL conical flask,10.0% inoculation level,agitation speed of 150 r/min,culture at 28 ℃ for 9 days. Effects of solid–liquid ratio,extraction time,extraction temperature and ultrasonic power on polysaccharide extraction were examined by orthogonal design,the best extraction process was solid-liquid ratio of 1∶75(g/mL),extraction temperature of 80 ℃,and extraction time of 60 min. Under these combined optimal conditions,the polysaccharide yield reached 5.7%.