中国食品添加剂

为了研究云南小粒咖啡果皮色素最佳提取、纯化工艺和抗氧化活性，通过响应面法优化试验和动态吸附-解吸试验确定色素的最佳提取、纯化工艺，考察粗色素及纯化后色素对DPPH·、ABTS⁺·和·OH的清除能力。结果表明云南小粒咖啡果皮色素的最佳提取工艺：乙醇体积分数52%、超声功率241 W、超声时间48 min、液料比55 mL/g，在此条件下提取率达到74.47%。采用X-5大孔树脂的最佳纯化条件：上样浓度6 mg/mL、上样流速2 mL/min，解吸液乙醇体积分数60%、解吸流速3 mL/min，该条件下色素纯化效率为55.38%。粗色素清除DPPH·、ABTS⁺·和·OH的IC₅₀值分别为(0.1788±0.0124)、(0.4084±0.0115)、(0.2492±0.0152)mg/mL；纯化后色素清除DPPH·、ABTS⁺·和·OH的IC₅₀值分别为(0.0774±0.0007)、(0.1220±0.0047)、(0.0963±0.0114) mg/mL。云南小粒咖啡果皮色素具有良好的抗氧化性，且纯化后色素抗氧化能力显著增强。研究结果为云南小粒咖啡果皮色素的进一步加工提供了理论依据。

In order to study the optimal extraction and purification process and antioxidant activity of the pigments from the pericarp of Yunnan Coffea arabica,the optimal extraction and purification process of the pigments was determined by response surface method optimization test and dynamic adsorption-desorption test,and the scavenging ability of the crude pigments and the purified pigments against DPPH·,ABTS⁺ · and ·OH was investigated. The results showed that the optimal extraction process for the pigment of the pericarp of Yunnan Coffea arabica was :ethanol volume fraction of 52%,ultrasonic power of 241 W,ultrasonic time of 48 min,and liquid-to-material ratio of 55 mL/g,and the extraction rate reached 74.47% under these conditions. The optimal purification conditions using X-5 microporous resin were :up-sample concentration of 6 mg/mL,up-sample flow rate of 2 mL/min,desorption solution ethanol volume fraction of 60%,desorption flow rate of 3 mL/min,and the pigment purification efficiency was 55.38% under these conditions. The IC₅₀ values for the crude pigment in scavenging DPPH·,ABTS⁺ · and ·OH were(0.1788±0.0124),(0.4084±0.0115),and(0.2492±0.0152) mg/mL,respectively;The IC₅₀ values for the purified pigment in scavenging DPPH·,ABTS⁺· and ·OH were(0.0774±0.0007),(0.1220±0.0047) and(0.0963±0.0114) mg/mL,respectively. The pigments extracted from Yunnan Coffea arabica pericarp had good antioxidant properties,and the antioxidant capacity of the purified pigments was significantly enhanced. The results provide a theoretical basis for the further processing of Yunnan Coffea arabica pericarp pigments.