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对酸浆宿萼中叶黄素的提取工艺进行研究。以正己烷为溶剂,超声波辅助提取法,考察提取时间、超声功率和料液比对提取率的影响,采用响应面法对其进行优化。结果表明:最佳工艺为提取时间35min,超声功率430W,料液比1∶37,叶黄素的提取率为8.19%。酸浆宿萼中叶黄素提取物清除DPPH·和·OH的半抑制质量浓度IC50分别为0.2195mg/mL和0.4031mg/mL,抗氧化活性较高,可作为一种良好的天然抗氧化剂。该工艺可用于酸浆宿萼中叶黄素提取的工业生产。
Abstract：Ultrasonic was used in the extraction of lutein from calyx of Physalis. Effects of main parameters including extraction time,ultrasonic power and solid ratio on extraction yields of lutein from calyx of Physails were studies.Based on single factor experimental analysis,through the response surface,the optimization parameters were obtained. The results indicated that the optimum technological conditions were:extraction time 35 min,solid to liquid ratio 1∶37,ultrasonic power 430 W. Under the above conditions,the yield of lutein was 8.19% and is very close to the predicated value. The half-inhibitory concentration IC50 of DPPH · and · OH in the total lutein extracted from the calyx of Physalis is 0.2195 mg/mL and 0.4031 mg/m L,respectively. It has high antioxidant activity and can be used as a good natural antioxidant. This study provides a reference for efficient extraction of lutein and the comprehensive utilization of calyx of Physalis.
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基本信息:
DOI：10.19804/j.issn1006-2513.2022.01.010
中图分类号:TS202.3
引用信息:
[1]罗维巍,李双,刁全平,等.响应面法优化超声提取酸浆宿萼中叶黄素的工艺及抗氧化活性研究[J].中国食品添加剂,2022,33(01):62-68.DOI:10.19804/j.issn1006-2513.2022.01.010.
基金信息:
辽宁省科技厅项目（SYSF202012,SYSF202004）; 鞍山师范学院校级项目
2021-07-02
2021
2021-09-26
2021
1
2022-01-20
2022-01-20
2022-01-20