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本研究在前期工作中系统比较了多种检测器对唾液酸乳糖（SL）的分离效果，结果表明电雾式检测器（CAD）在通用性、兼容性和响应一致性等方面均表现出显著优势。基于此，首次使用超高效液相色谱-电雾式检测器（UPLC-CAD）开发了SL生产工艺中6种关键糖类物质的同步、快速和准确定量检测方法，有效克服了传统手段响应低、前处理繁琐或无法同步检测等局限。选用BEH Amide色谱柱分离，以20 mmol/L甲酸铵溶液和乙腈作为流动相进行梯度洗脱，结果显示：各物质重现性好，方法精密度高（RSD<2%），且目标物回收率稳定（94%〜101%）。综上，该方法可有效分离分析乳糖（LA），唾液酸（SA）、3'-唾液酸乳糖（3'-SL）、6'-唾液酸乳糖（6'-SL）、3'-唾液酸乳果糖（3'-SLu）和6'-唾液酸乳果糖（6'-SLu），为生产工艺监控与产品质量评估提供了可靠的技术支撑。
Abstract：In this study,various detection systems were systematically evaluated for their effectiveness in analyzing sialyllactose(SL). Preliminary results indicated that the charged aerosol detector(CAD) exhibited significant advantages in terms of versatility,compatibility,and response consistency. Consequently,an ultra-performance liquid chromatography–charged aerosol detector(UPLC-CAD) method was developed for the first time to enable the simultaneous,rapid,and accurate quantification of six key sugar impurities in the SL production process. This method effectively overcame the limitations of traditional approaches,such as low response,cumbersome sample preparation,or inability to perform simultaneous detection. Chromatographic separation was achieved using a BEH Amide column with a gradient elution using 20 mmol/L ammonium formate aqueous solution and acetonitrile. The results demonstrated good reproducibility,high method precision(RSD<2%),and stable analyte recoveries(94%〜101%). In summary,this method could effectively separate and analyze lactose(LA),sialic acid(SA),3'-sialyllactose(3'-SL),6'-sialyllactose(6'-SL),3'-sialyl-lactulose(3'-SLu),and 6'-sialyl-lactulose(6'-SLu),providing reliable technical support for process monitoring and product quality evaluation.
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基本信息:
DOI：10.19804/j.issn1006-2513.2026.5.016
中图分类号:TS252.1
引用信息:
[1]舒敏,徐柳柳,杨丹露,等.唾液酸乳糖及其相关杂糖的快速分析方法研究[J].中国食品添加剂,2026,37(05):134-143.DOI:10.19804/j.issn1006-2513.2026.5.016.
基金信息:
功能营养素合成生物绿色制造关键技术攻关项目(No.2023020302020708)
2025-09-23
2025
2025-11-19
2025-11-20
2025
1
2026-05-15
2026-05-15
2026-05-15